human angiogenesis array c1  (RayBiotech inc)

Journal: Stem Cell Research & Therapy

Article Title: Human platelet lysate-cultured adipose-derived stem cell sheets promote angiogenesis and accelerate wound healing via CCL5 modulation

doi: 10.1186/s13287-024-03762-9

Figure Lengend Snippet: Enhanced expression of CCL5 and angiogenin in HPL sheets. ( A ) Angiogenesis-related proteins in the conditioned medium of FBS or HPL sheets were analyzed by Human Angiogenesis Array C1. Representative images of angiogenesis array and the indicated map were shown. Red boxes marked out CCL5 and blue boxes indicated angiogenin. ( B ) The secretion level of each protein was normalized by reference spots. The results revealed significantly enhanced expression of CCL5 and angiogenin in HPL sheet group ( n = 3 samples/condition, ** p < 0.01, *** p < 0.001 relative to the FBS group, multiple t test). ( C ) Quantitative PCR measurements for CCL5 and angiogenin in FBS and HPL sheets ( n = 3 samples/condition; * p < 0.05 and *** p < 0.001 relative to FBS sheet group, Student’s t test). ( D ) Release of CCL5 and angiogenin from FBS sheets and HPL sheets was determined by ELISA. Conditioned medium (CM) was from 3 independent batches ( n = 9 samples/condition, *** p < 0.001 relative to the FBS sheet group, Student’s t test). Values are presented as means ± SD. FBS, fetal bovine serum; HPL, human platelet lysate; EGF, epidermal growth factor; CXCL5, C-X-C motif chemokine ligand 5; FGF2, basic fibroblast growth factor 2; GRO, growth-regulated oncogene; IFN-gamma, interferon-gamma; IGF-1, insulin growth factor-1; IL-6, interleukin-6; IL-8, interleukin-8; MCP-1, monocyte chemoattractant protein-1; PDGF-BB, platelet-derived growth factor subunit B; PlGF, placenta growth factor; CCL5, C-C motif chemokine ligand 5; TGF-beta1, transforming growth factor-beta 1; TIMP, tissue inhibitor matrix metalloproteinase; TPO, thrombopoietin; VEGF, vascular epidermal growth factor; CM, conditioned medium

Article Snippet: Analysis of secretion profile in the conditioned medium of FBS or HPL-cultured ASC sheets was performed using a Human Angiogenesis Array C1 (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions, allowing semi-quantitative determination of protein levels of a variety of pro-angiogenic factors.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Oncology Letters

Article Title: Circular dumbbell miR-34a-3p and −5p suppresses pancreatic tumor cell-induced angiogenesis and activates macrophages

doi: 10.3892/ol.2020.12336

Figure Lengend Snippet: Angiogenesis array results. A significant increase of CCL5 expression was detected in (A) PANC-1 (P=0.0028) and (B) MIA PaCa-2 cell lines (P=0.0034) for db34a-transfected cells vs. dbSCR-transfected control cells. *P≤0.05, **P≤0.01. (C) RayBio Human Angiogenesis Array C1 map. CCL5, C-C motif chemokine ligand 5; db34a, miR-34a-3p and −5p dumbbell RNA; dbSCR, scrambled dumbbell RNA.

Article Snippet: An angiogenic antibody array analysis was performed using a RayBio ® Human Angiogenesis Array C1 kit (AAH-ANG-1; RayBiotech, Inc.) according to the manufacturer's instructions.

Techniques: Expressing, Transfection

Journal: Oncology Letters

Article Title: Circular dumbbell miR-34a-3p and −5p suppresses pancreatic tumor cell-induced angiogenesis and activates macrophages

doi: 10.3892/ol.2020.12336

Figure Lengend Snippet: Angiogenesis array results. A significant increase of CCL5 expression was detected in (A) PANC-1 (P=0.0028) and (B) MIA PaCa-2 cell lines (P=0.0034) for db34a-transfected cells vs. dbSCR-transfected control cells. *P≤0.05, **P≤0.01. (C) RayBio Human Angiogenesis Array C1 map. CCL5, C-C motif chemokine ligand 5; db34a, miR-34a-3p and −5p dumbbell RNA; dbSCR, scrambled dumbbell RNA.

Article Snippet: To evaluate the expression of pro-angiogenic molecules, the RayBio Human Angiogenesis Array C1 kit was used.

Techniques: Expressing, Transfection